Publications
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Bozhüyük KAJ, Präve L, Kegler C, Schenk L, Kaiser S, Schelhas C, Shi YN, Kuttenlochner W, Schreiber M, Kandler J, Alanjary M, Mohiuddin TM, Groll M, Hochberg GKA, Bode HB. Evolution-inspired engineering of nonribosomal peptide synthetases. Science. 2024 Mar 22;383(6689):eadg4320. doi: 10.1126/science.adg4320. Epub 2024 Mar 22. PMID: 38513038.
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Bozhüyük KAJ, Präve L, Kegler C, Kaiser S, Shi YN, Kuttenlochner W, Schenk L, Mohiuddin TM, Groll M, Hochberg GKA, Bode HB. Evolution Inspired Engineering of Megasynthetases. bioRxiv 2022.12.02.518901; doi: https://doi.org/10.1101/2022.12.02.518901
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Abbood N, Präve L, Bozhueyuek KAJ, Bode HB. A Practical Guideline to Engineering Nonribosomal Peptide Synthetases. Methods Mol Biol. 2023;2670:219-234. doi: 10.1007/978-1-0716-3214-7_11. PMID: 37184707.
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Abbood N, Duy Vo T, Watzel J, Bozhueyuek KAJ, Bode HB. Type S Non-Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries. Chemistry. 2022 May 6;28(26):e202103963. doi: 10.1002/chem.202103963. Epub 2022 Mar 29. PMID: 35176184; PMCID: PMC9315016.
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Koch P, Schmitt S, Cardner M, Beerenwinkel N, Panke S, Held M. Discovery of antimicrobials by massively parallelized growth assays (Mex). Sci Rep. 2022 Mar 8;12(1):4097. doi: 10.1038/s41598-022-07755-7. PMID: 35260685; PMCID: PMC8904554.
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Bozhueyuek KAJ, Watzel J, Abbood N, Bode HB. Synthetic Zippers as an Enabling Tool for Engineering of Non-Ribosomal Peptide Synthetases*. Angew Chem Int Ed Engl. 2021 Aug 2;60(32):17531-17538. doi: 10.1002/anie.202102859. Epub 2021 Jun 27. PMID: 34015175; PMCID: PMC8362031.
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Napiorkowska M, Pestalozzi L, Panke S, Held M, Schmitt S. High-Throughput Optimization of Recombinant Protein Production in Microfluidic Gel Beads. Small. 2021 Jan;17(2):e2005523. doi: 10.1002/smll.202005523. Epub 2020 Dec 16. PMID: 33325637.
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Tietze A, Shi YN, Kronenwerth M, Bode HB. Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains. Chembiochem. 2020 Oct 1;21(19):2750-2754. doi: 10.1002/cbic.202000176. Epub 2020 Jun 25. PMID: 32378773; PMCID: PMC7586950.
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Bozhüyük KA, Micklefield J, Wilkinson B. Engineering enzymatic assembly lines to produce new antibiotics. Curr Opin Microbiol. 2019 Oct;51:88-96. doi: 10.1016/j.mib.2019.10.007. Epub 2019 Nov 16. PMID: 31743841; PMCID: PMC6908967.
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Jusková P, Schmid YRF, Stucki A, Schmitt S, Held M, Dittrich PS. "Basicles": Microbial Growth and Production Monitoring in Giant Lipid Vesicles. ACS Appl Mater Interfaces. 2019 Sep 25;11(38):34698-34706. doi: 10.1021/acsami.9b12169. Epub 2019 Sep 10. PMID: 31454223; PMCID: PMC7462352.
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Kegler C, Bode HB. Artificial Splitting of a Non-Ribosomal Peptide Synthetase by Inserting Natural Docking Domains. Angew Chem Int Ed Engl. 2020 Aug 3;59(32):13463-13467. doi: 10.1002/anie.201915989. Epub 2020 May 27. PMID: 32329545; PMCID: PMC7496407.
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Bozhüyük KAJ, Linck A, Tietze A, Kranz J, Wesche F, Nowak S, Fleischhacker F, Shi YN, Grün P, Bode HB. Modification and de novo design of non-ribosomal peptide synthetases using specific assembly points within condensation domains. Nat Chem. 2019 Jul;11(7):653-661. doi: 10.1038/s41557-019-0276-z. Epub 2019 Jun 10. PMID: 31182822.
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Schmitt S, Montalbán-López M, Peterhoff D, Deng J, Wagner R, Held M, Kuipers OP, Panke S. Analysis of modular bioengineered antimicrobial lanthipeptides at nanoliter scale. Nat Chem Biol. 2019 May;15(5):437-443. doi: 10.1038/s41589-019-0250-5. Epub 2019 Apr 1. PMID: 30936500.
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Bozhüyük KAJ, Fleischhacker F, Linck A, Wesche F, Tietze A, Niesert CP, Bode HB. De novo design and engineering of non-ribosomal peptide synthetases. Nat Chem. 2018 Mar;10(3):275-281. doi: 10.1038/nchem.2890. Epub 2017 Dec 11. PMID: 29461518.
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Meyer A, Pellaux R, Potot S, Becker K, Hohmann HP, Panke S, Held M. Optimization of a whole-cell biocatalyst by employing genetically encoded product sensors inside nanolitre reactors. Nat Chem. 2015 Aug;7(8):673-8. doi: 10.1038/nchem.2301. Epub 2015 Jul 13. PMID: 26201745.